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Science Laboratory Prep Schedule

Bio I

Michele Snyder

 

 January 2009
Monday Tuesday Wednesday Thursday Friday
      1
2
5
6
7  
8
9
12
13
14
15
16
19
20
21
22
23
26
27
28
Safety, Metric Measurement, Microscope Anatomy
29 30

 

 

 February 2009
Monday Tuesday Wednesday Thursday Friday
2
3
4
Scientific Inq./ Sci. Method
Exp. Design
5
6
9
10
11
Scientific Inq.
12
13
16
17
18
Microscopy
19
20
23
24
25
Transport
26
27

       

 

 

 March 2009
Monday Tuesday Wednesday Thursday Friday
2
3
4
Enzymes: Lactose Intol. I
5
6
9
10
11
Enzymes: Lactose Intol. II
12
13
16 17 18 19 20
23
24
25
Photosynth. part I:
Intro to Spectro
26
27
30
31
     

 

 

 April 2009
Monday Tuesday Wednesday Thursday Friday
    1
Photosynth. part II:
pH and Photosynthesis
2
3
6
7
8
Cancer Gene Detect. I
9
10
13
14
15
Cancer Gene Detect. II
16
17
20
21
22
Mendelian Genet./ Yeast Genetics
23
24
27
28
29
Yeast Genetics: Data Analysis
30

 

 

 May 2009
Monday Tuesday Wednesday Thursday Friday
        1
4
5
6
Bacterial Transformation
7
8
11
12
13
Bacterial Transformation II
14
15
18
19
20
21
22
25
26
27
28
29

 

 

 

Lab Title

Prep Materials

Safety, Metric Measurement and Intro to Microscopy Part I
- Various Slides
- slides and coverslips
- Yogurt
part II:
- Onion
- Elodea
- Potato
- IKI ( iodine solution) 
- Toothpicks ( wrapped)
- Methylene Blue stain
- Baker's Yeast
- Hay Infusion
- Please click to make changes**
Microscopy and Scientific Method
Exp. Design w/ Euglena
- Euglena Media ( sterilized )
- Live Euglena ( live delivery over weeks of labs )
- Orb Shaker
- Elodea
- Please click to make changes
**
Macromolecules  1% Glucose
1% Sucrose
1-2% Starch
1% Albumin, 
1-2% Pepsin, 
Benedict's solution
IKI solution
Vegetable Oil
0.2% HCl
Biuret ( 3% CuSO4; 10% KOH)
Other materials: 
Onion, Potato, Brown Paper squares
Rubber stoppers

- Please click to make changes**
Cells and Transport
- 10% Starch Soln
- 10% NaCl Soln
- 10% Glucose Soln
- 10% Albumin  Soln
- Elodea
- Please click to make changes
**
Enzymes: Lactase - lactose intolerance Lactaid Fast Act
Milks:
 - Cow, Soy Rice
Glucose Test Strips 100/ lab
pH solns:
 - pH 2,4,7,10 and 12 ( made with HCl and NaOH )
Short test tubes ( screw top culture tubes )
 - racks to fit them
Plastic 1L beakers
Mortar and Pestle ( 4 min )
Conical centrifuge tubes ( plastic)
Water bath ( boiling ) 
ICE
- Please click to make changes**
Photosynthesis prt I Spinach in Bag
Phosphate Buffer (per liter )
 - 8.70g K-phosphate diabasic
 - 6.80g K-phosphate monobasic
80% Acetone aloquats (10ml)

- Please click to make changes
**
Photosynthesis prt II Elodea
pH buffers 4 and 7 for calibration ( optional )
- Please click to make changes
**
Mitosis and Cell Cycle - Please click to make changes**
Mendelian Genetics  - Please click to make changes**
Mendelian Genetics Yeast  Yeast strains (9): 
 - HA2
 - HBT
 - HAO
 - HAR
 - HAT
 - HART
 - HBO
 - HBR
 - HBRT
Media:
- YED plates 30 / class
- MVA plates 5 / class
- MV plates 5 / class
- Please click to make changes**
DNA Finger Printing / Blood Typing Edvotek Kit # 109:
- make Buffer dilution
- pour gels 
- set up E-phoresis app.
Blood Typing Kit ( synth. ABO )
- Please click to make changes**
Bact Trans BioRad Kit P-Glo Trans
- make Plates 1 LB/ 2 LB-amp/ 1 LB-amp-arabinose per group
- 24 hours ahead - grow E. coli
- aliquot broth and tran. soln 
- check water bath temp
- ice on day of lab
- Please click to make changes
**
Transcription & Translation - Please click to make changes**
Cancer Gene Detect. I  Gels for Electrophoresis

- Please click to make changes**

  - Please click to make changes**
  - Please click to make changes**
  - Please click to make changes**
Osmoregulation - Vegetable cubes
- 1%, 5%, 10% KMnO4 solutions
- Vernier Calipers
- Stopwatch or Clock
- 10% Starch Soln
- 10% NaCl Soln
- 10% Glucose Soln
- 10% Albumin  Soln
- 0.9% NaCl Soln
- 0.1% NaCl Soln
- 5% glucose Soln
- 1% glucose Soln
- Please click to make changes
**
Enzymes: Catalase 100mM Phosphate Buffer 
 ( 1 liter - 8.70 K2HPO4 dibasic; 6.80 KH2PO4 )
- filter paper discs ( many )
- Stop Watches
- H2O2 ( 0.3%, 0.6%, 0.8%, 1.0%, 1.5%, 2.0%)
- glass vials (approx 50 ml)
- 10 ml dispenser 
- Blender
- Ice
- Aluminum Foil
- Potatoes
- Please click to make changes
**

 

Contact info:

Room 221 Stafford Center for Art, Science and Technology
Clinton Community College
136 Clinton Pt. Dr.
Plattsburgh, NY 12901
                                                                   518-562-4337

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